Related Services

Paternity Testing - Dr. Colucci

  1. DNA analysis for paternity identification/forensic medicine fingerprinting: DNA isolated from peripheral blood or body tissues/fluids is analyzed using a comprenhensive panel of informative microsatellite markers
  2. Consultations for paternity disputes: Assist in all the steps, technical and legal, involved with litigation/court issues
  3. Protocols and applications for Molecular Medicine: Identify the best molecular tools which could contribute to a more effective clinical diagnosis, prognosis and monitoring of disease states; assist in the selection/interpretattion of molecular data in a clinical setting



1) Paternity testing

AKonsult can provide for the DNA analysis for paternity identification and forensic medicine DNA fingerprinting analyses. The DNA is isolated from peripheral blood or body tissues/fluids and is analysed in our partner laboratories in Switzerland and Italy making use of a comprehensive panel of informative microsatellite markers and interpretation tools. In a typical case, peripheral blood is taken by conventional venipuncture from all the interested parties, mother, child and alleged father(s), either in one of our reference laboratories or blood can be taken by a professional nurse and mailed to us. Subsequently, it is processed for DNA isolation and analysis. In a 3-4 weeks time frame, the individual DNA profiles are generated and compared between mother and child, and child and alleged father(s). The individual marker alleles, which the child has not inherited from the mother, are looked for in the DNA from the alleged father(s). Depending on whether or not a match is found, paternity is excluded or confirmed. In this case the probability of paternity for the alleged father is calculated using the adequate statistical tools and a detailed report prepared and issued. From the first meeting with Dr. Giuseppe Colucci it will take 8 weeks to get a reliable response to the pressing question of paternity.

AKonsult can provide for a Consultation Service in the context of paternity disputes, covering all the steps, laboratory, technical and legal, to be undertaken in litigation cases with respect to paternity. This includes defining an a priori probability of paternity of alleged father(s), identifying the proper type of action with regard to when and how perform the tests, work in close contact with a given Court's technical advisers and laboratories. Dr. Giuseppe Colucci can be appointed as expert witness for an involved party and critically assess the paternity testing procedures performed in order to ensure the integrity of the results obtained by the court's appointed laboratories for a given involved party or individual.

Background information on Paternity Testing

The development of sensitive nucleic acid (DNA/RNA) detection techniques and the identification of highly polymorphic genetic markers has revolutionised the area of paternity testing and of forensic medicine. DNA fingerprinting, that is the possibility of typing individuals at the genetic level, has become the method of choice for solving paternity issues or disputes, and it has been established as well as the tool to identify (and relating to a given suspect) the biological traces found f.e. at a the scene of crime. For both applications, DNA is isolated from venous blood or other types of tissue or body fluids and is analysed with several genetic markers, usually consisting of mini- (VNTR) or microsatellites (STR). These are sequences that differ by a variable number of repeated nucleotides. They can be separated into a variety of different molecular forms known as alleles and hence allow the identification of individual specific patterns.

A combined genetic profile can thus be obtained. It may, or may not, match with that of either the alleged father or, in forensic applications, of the crime suspect. The probability of a match (or of paternity) is calculated by using statistic formulas and this taken as a decision base to confirm or exclude a so-called significant match. With the currently available VNTR or STR markers it is possible to reach probabilities close to 100% (99,99999%) with a high degree of confidence in the results. Several effective quality control procedures are used, including retesting a previously typed DNA samples, adding specific allele ladders and amplifying X and Y chromosome markers for sex identification.

Should you wish to obtain further information, please fill out the form provided in "Contact us"

Some key references

  • Lobbiani A., Nocco A., Vedrietti P., Brambati B., Colucci G. Prenatal paternity testing by DNA analysis. Prenatal Diagnosis 11:343-346, 1991.
  • Terzoli G., Lalatta F., Lobbiani A., Simoni G., Colucci G. Fertility in a 47,XXY patient: assessment of biological paternity by deoxyribonucleic acid fingerprinting. Fertility and Sterility 58:821-822, 1992.
  • Hantschel M, Hausmann R, Lederer T, Martus P, Betz P.Population genetics of nine short tandem repeat (STR) loci - DNA typing using the AmpFlSTR profiler PCR amplification kit. Int J Legal Med 1999;112(6):393-5


2) Molecular medicine application support.

AKonsult can identify the best molecular tools which can contribute to a more effective clinical diagnosis, prognosis and monitoring of disease states; thus, we can assist in the selection and interpretation of molecular data in a clinical setting. Based on an expertise developed over many years of pioneering work in this field, Dr. Giuseppe Colucci can provide advice in the definition of clinical indications for molecular tests and identify the proper applications and laboratory procedures, as well as and the most reliable molecular reference laboratories, and review and interpret your data.

Background information or molecular medicine application support

Molecular medicine is a new term used more and more often to define the application of molecular biology techniques to the study of the pathogenetic mechanism of human diseases. Prompted by the human genome project, new technologies and applications have been developed that facilitate the identification of hundreds of genes associated with mono- and polygenic inheritable diseases. In addition to genetic disorders, such as cystic fibrosis, thalassemia and polycystic kidney disease, more complex diseases, including Alzheimer, diabetes and myocardial infarction, have been linked to specific gene mutations or HLA polymorphisms. This has opened new research avenues for a better understanding of disease pathogenesis, and to identify pre-symptomatic individuals at risk. One of the major objectives is the development of gene therapy procedures to correct the original genomic alterations.

Moreover, the analysis of these gene products and their interplay with the target cell(s) is a new tool pharmaceutical companies are using to develop new and more specific therapeutic agents to increase the effectiveness of the therapy management.

Several diagnostic tests are already used to identify carriers of a given genetic disease. In prenatal diagnosis, these tests are used to define genetic profiles (haplotypes) of individuals at risk of contracting certain chronic or neoplastic diseases.

See for further reference for example

  • Brown P. O., Hartwell L. Genomics and human disease-variations on variation. Nature Genetics 18:91-93, 1998.